Western Blot:Article Title: Autocrine interferon poisoning mediates ADAR1-dependent synthetic lethality in BRCA1/2 -mutant cancers
Article Snippet: Target Epitope specificity Application Manufacturer Reference Working dilution ADAR1 p110, p150 WB Cell Signaling Technologies #14175 1:1,000 p110, p150 IHC Santa Cruz #sc-73408 1:700 p150 IHC Bethyl Laboratories #A303-883A 1 :1,000 β-Actin WB Sigma Aldrich #A1978 1:10,000 BRCA1 WB Cell Signaling Technologies #9010 1:1,000 BRCA2 WB Cell Signaling Technologies #10741 1:1,000 Casp3 WB Cell Signaling Technologies #9668 1:1,000 c-Casp3 N175 WB Cell Signaling Technologies #9661 1:1,000 CCNA2 IF Abcam #ab181591 1:1,000 CGAS WB Cell Signaling Technologies #15102 1:1,000 CHK1 WB Cell Signaling Technologies #2360 1:1,000 p-CHK1 S345 WB Cell Signaling Technologies #2341 1:1,000 eIF2α WB Cell Signaling Technologies #5324 1:1,000 p-eIF2α S51 WB Cell Signaling Technologies #3398 1:1,000 Fibrillarin IF Abcam #ab166630 1:4,000 G3BP1 WB, IF Cell Signaling Technologies #61559 1:1,000 GAPDH WB Cell Signaling Technologies #2118 1:1,000 γ-H2AX S139 WB, IF Merck #05-636 1:1,000 IRF3 WB (Hs) Abcam #ab76409 1:1,000 WB (Mm) Cell Signaling Technologies #4302 1:1,000 p-IRF3 S386 WB (Hs) Abcam #ab76493 1:1,000 S396 WB (Mm) Cell Signaling Technologies #29047 1:1,000 LGP2 WB (Hs) Cell Signaling Technologies #12869 1:1,000 WB (Mm) Abcam #67270 1:1,000 MDA5 WB Cell Signaling Technologies #5321 1:1,000 c-PARP1 N214 WB Cell Signaling Technologies #9541 1:1,000 Target Epitope specificity Application Manufacturer Reference Working dilution PKR WB Cell Signaling Technologies #12297 1:1,000 IF Santa Cruz #sc-6282 1:500 p-PKR T446 WB Abcam #ab32036 1:1,000 RAD51 IF Abcam #ab133534 1:1,000 RIG1 WB Cell Signaling Technologies #3743 1:1,000 RNase H1 WB Santa Cruz #sc-376326 1:500 RNA:DNA hybrids IF Kerafast #ENH001 1:500 RPA IF Abcam #ab2175 1:1,000 STAT1 WB (Hs) Cell Signaling Technologies #9176 1:1,000 WB (Mm) Cell Signaling Technologies #14994 1:1,000 p-STAT1 T701 WB Cell Signaling Technologies #9167 1:1,000 Supplementary Table 2.
Article Title: Autocrine interferon poisoning mediates ADAR1-dependent synthetic lethality in BRCA1/2 -mutant cancers
Article Snippet: ADAR1 (p110, p150) - WB - Cell Signaling Technologies #14175 1:1,000 ADAR1 (p110, p150) - IHC - Santa Cruz #sc-73408 1:700 ADAR1 (p150) - IHC - Bethyl Laboratories #A303-883A 1 :1,000 -Actin - WB - Sigma Aldrich #A1978 1:10,000 BRCA1 - WB - Cell Signaling Technologies #9010 1:1,000 BRCA2 - WB - Cell Signaling Technologies #10741 1:1,000 Casp3 - WB - Cell Signaling Technologies #9668 1:1,000 c-Casp3 (N175) - WB - Cell Signaling Technologies #9661 1:1,000 CCNA2 - IF - Abcam #ab181591 1:1,000 CGAS - WB - Cell Signaling Technologies #15102 1:1,000 CHK1 - WB - Cell Signaling Technologies #2360 1:1,000 p-CHK1 (S345) - WB - Cell Signaling Technologies #2341 1:1,000 eIF2 - WB - Cell Signaling Technologies #5324 1:1,000 p-eIF2 (S51) - WB - Cell Signaling Technologies #3398 1:1,000 Fibrillarin - IF - Abcam #ab166630 1:4,000 G3BP1 - WB, IF - Cell Signaling Technologies #61559 1:1,000 GAPDH - WB - Cell Signaling Technologies #2118 1:1,000 -H2AX (S139) - WB, IF - Merck #05-636 1:1,000 IRF3 - WB (Hs) - Abcam #ab76409 1:1,000 IRF3 - WB (Mm) - Cell Signaling Technologies #4302 1:1,000 p-IRF3 (S396) - WB (Hs) - Abcam #ab76493 1:1,000 p-IRF3 (S396) - WB (Mm) - Cell Signaling Technologies #29047 1:1,000 LGP2 - WB (Hs) - Cell Signaling Technologies #12869 1:1,000 LGP2 - WB (Mm) - Abcam #67270 1:1,000 MDA5 - WB - Cell Signaling Technologies #5321 1:1,000 c-PARP1 (N214) - WB - Cell Signaling Technologies #9141 1:1,000 PKR - WB - Cell Signaling Technologies #12297 1:1,000 PKR - IF - Santa Cruz #sc-6282 1:500 p-PKR (T446) - WB - Abcam #ab32036 1:1,000 RAD51 - IF - Abcam #ab133534 1:1,000 RIG1 - WB - Cell Signaling Technologies #3743 1:1,000 RNase H1 - WB - Santa Cruz #sc-376326 1:500 RNA:DNA hybrids - IF - Kerafast #ENH001 1:500 RPA - IF - Abcam #ab2175 1:1,000 STAT1 - WB (Hs) - Cell Signaling Technologies #9176 1:1,000 STAT1 - WB (Mm) - Cell Signaling Technologies #14994 1:1,000 p-STAT1 (T701) - WB - Cell Signaling Technologies #9167 1:1,000 Unless indicated otherwise, all antibodies used in this study were validated externally by the suppliers for each corresponding application.
Immunohistochemistry:Article Title: Autocrine interferon poisoning mediates ADAR1-dependent synthetic lethality in BRCA1/2 -mutant cancers
Article Snippet: Target Epitope specificity Application Manufacturer Reference Working dilution ADAR1 p110, p150 WB Cell Signaling Technologies #14175 1:1,000 p110, p150 IHC Santa Cruz #sc-73408 1:700 p150 IHC Bethyl Laboratories #A303-883A 1 :1,000 β-Actin WB Sigma Aldrich #A1978 1:10,000 BRCA1 WB Cell Signaling Technologies #9010 1:1,000 BRCA2 WB Cell Signaling Technologies #10741 1:1,000 Casp3 WB Cell Signaling Technologies #9668 1:1,000 c-Casp3 N175 WB Cell Signaling Technologies #9661 1:1,000 CCNA2 IF Abcam #ab181591 1:1,000 CGAS WB Cell Signaling Technologies #15102 1:1,000 CHK1 WB Cell Signaling Technologies #2360 1:1,000 p-CHK1 S345 WB Cell Signaling Technologies #2341 1:1,000 eIF2α WB Cell Signaling Technologies #5324 1:1,000 p-eIF2α S51 WB Cell Signaling Technologies #3398 1:1,000 Fibrillarin IF Abcam #ab166630 1:4,000 G3BP1 WB, IF Cell Signaling Technologies #61559 1:1,000 GAPDH WB Cell Signaling Technologies #2118 1:1,000 γ-H2AX S139 WB, IF Merck #05-636 1:1,000 IRF3 WB (Hs) Abcam #ab76409 1:1,000 WB (Mm) Cell Signaling Technologies #4302 1:1,000 p-IRF3 S386 WB (Hs) Abcam #ab76493 1:1,000 S396 WB (Mm) Cell Signaling Technologies #29047 1:1,000 LGP2 WB (Hs) Cell Signaling Technologies #12869 1:1,000 WB (Mm) Abcam #67270 1:1,000 MDA5 WB Cell Signaling Technologies #5321 1:1,000 c-PARP1 N214 WB Cell Signaling Technologies #9541 1:1,000 Target Epitope specificity Application Manufacturer Reference Working dilution PKR WB Cell Signaling Technologies #12297 1:1,000 IF Santa Cruz #sc-6282 1:500 p-PKR T446 WB Abcam #ab32036 1:1,000 RAD51 IF Abcam #ab133534 1:1,000 RIG1 WB Cell Signaling Technologies #3743 1:1,000 RNase H1 WB Santa Cruz #sc-376326 1:500 RNA:DNA hybrids IF Kerafast #ENH001 1:500 RPA IF Abcam #ab2175 1:1,000 STAT1 WB (Hs) Cell Signaling Technologies #9176 1:1,000 WB (Mm) Cell Signaling Technologies #14994 1:1,000 p-STAT1 T701 WB Cell Signaling Technologies #9167 1:1,000 Supplementary Table 2.
Article Title: Autocrine interferon poisoning mediates ADAR1-dependent synthetic lethality in BRCA1/2 -mutant cancers
Article Snippet: ADAR1 (p110, p150) - WB - Cell Signaling Technologies #14175 1:1,000 ADAR1 (p110, p150) - IHC - Santa Cruz #sc-73408 1:700 ADAR1 (p150) - IHC - Bethyl Laboratories #A303-883A 1 :1,000 -Actin - WB - Sigma Aldrich #A1978 1:10,000 BRCA1 - WB - Cell Signaling Technologies #9010 1:1,000 BRCA2 - WB - Cell Signaling Technologies #10741 1:1,000 Casp3 - WB - Cell Signaling Technologies #9668 1:1,000 c-Casp3 (N175) - WB - Cell Signaling Technologies #9661 1:1,000 CCNA2 - IF - Abcam #ab181591 1:1,000 CGAS - WB - Cell Signaling Technologies #15102 1:1,000 CHK1 - WB - Cell Signaling Technologies #2360 1:1,000 p-CHK1 (S345) - WB - Cell Signaling Technologies #2341 1:1,000 eIF2 - WB - Cell Signaling Technologies #5324 1:1,000 p-eIF2 (S51) - WB - Cell Signaling Technologies #3398 1:1,000 Fibrillarin - IF - Abcam #ab166630 1:4,000 G3BP1 - WB, IF - Cell Signaling Technologies #61559 1:1,000 GAPDH - WB - Cell Signaling Technologies #2118 1:1,000 -H2AX (S139) - WB, IF - Merck #05-636 1:1,000 IRF3 - WB (Hs) - Abcam #ab76409 1:1,000 IRF3 - WB (Mm) - Cell Signaling Technologies #4302 1:1,000 p-IRF3 (S396) - WB (Hs) - Abcam #ab76493 1:1,000 p-IRF3 (S396) - WB (Mm) - Cell Signaling Technologies #29047 1:1,000 LGP2 - WB (Hs) - Cell Signaling Technologies #12869 1:1,000 LGP2 - WB (Mm) - Abcam #67270 1:1,000 MDA5 - WB - Cell Signaling Technologies #5321 1:1,000 c-PARP1 (N214) - WB - Cell Signaling Technologies #9141 1:1,000 PKR - WB - Cell Signaling Technologies #12297 1:1,000 PKR - IF - Santa Cruz #sc-6282 1:500 p-PKR (T446) - WB - Abcam #ab32036 1:1,000 RAD51 - IF - Abcam #ab133534 1:1,000 RIG1 - WB - Cell Signaling Technologies #3743 1:1,000 RNase H1 - WB - Santa Cruz #sc-376326 1:500 RNA:DNA hybrids - IF - Kerafast #ENH001 1:500 RPA - IF - Abcam #ab2175 1:1,000 STAT1 - WB (Hs) - Cell Signaling Technologies #9176 1:1,000 STAT1 - WB (Mm) - Cell Signaling Technologies #14994 1:1,000 p-STAT1 (T701) - WB - Cell Signaling Technologies #9167 1:1,000 Unless indicated otherwise, all antibodies used in this study were validated externally by the suppliers for each corresponding application.
Recombinase Polymerase Amplification:Article Title: Autocrine interferon poisoning mediates ADAR1-dependent synthetic lethality in BRCA1/2 -mutant cancers
Article Snippet: Target Epitope specificity Application Manufacturer Reference Working dilution ADAR1 p110, p150 WB Cell Signaling Technologies #14175 1:1,000 p110, p150 IHC Santa Cruz #sc-73408 1:700 p150 IHC Bethyl Laboratories #A303-883A 1 :1,000 β-Actin WB Sigma Aldrich #A1978 1:10,000 BRCA1 WB Cell Signaling Technologies #9010 1:1,000 BRCA2 WB Cell Signaling Technologies #10741 1:1,000 Casp3 WB Cell Signaling Technologies #9668 1:1,000 c-Casp3 N175 WB Cell Signaling Technologies #9661 1:1,000 CCNA2 IF Abcam #ab181591 1:1,000 CGAS WB Cell Signaling Technologies #15102 1:1,000 CHK1 WB Cell Signaling Technologies #2360 1:1,000 p-CHK1 S345 WB Cell Signaling Technologies #2341 1:1,000 eIF2α WB Cell Signaling Technologies #5324 1:1,000 p-eIF2α S51 WB Cell Signaling Technologies #3398 1:1,000 Fibrillarin IF Abcam #ab166630 1:4,000 G3BP1 WB, IF Cell Signaling Technologies #61559 1:1,000 GAPDH WB Cell Signaling Technologies #2118 1:1,000 γ-H2AX S139 WB, IF Merck #05-636 1:1,000 IRF3 WB (Hs) Abcam #ab76409 1:1,000 WB (Mm) Cell Signaling Technologies #4302 1:1,000 p-IRF3 S386 WB (Hs) Abcam #ab76493 1:1,000 S396 WB (Mm) Cell Signaling Technologies #29047 1:1,000 LGP2 WB (Hs) Cell Signaling Technologies #12869 1:1,000 WB (Mm) Abcam #67270 1:1,000 MDA5 WB Cell Signaling Technologies #5321 1:1,000 c-PARP1 N214 WB Cell Signaling Technologies #9541 1:1,000 Target Epitope specificity Application Manufacturer Reference Working dilution PKR WB Cell Signaling Technologies #12297 1:1,000 IF Santa Cruz #sc-6282 1:500 p-PKR T446 WB Abcam #ab32036 1:1,000 RAD51 IF Abcam #ab133534 1:1,000 RIG1 WB Cell Signaling Technologies #3743 1:1,000 RNase H1 WB Santa Cruz #sc-376326 1:500 RNA:DNA hybrids IF Kerafast #ENH001 1:500 RPA IF Abcam #ab2175 1:1,000 STAT1 WB (Hs) Cell Signaling Technologies #9176 1:1,000 WB (Mm) Cell Signaling Technologies #14994 1:1,000 p-STAT1 T701 WB Cell Signaling Technologies #9167 1:1,000 Supplementary Table 2.
Article Title: Autocrine interferon poisoning mediates ADAR1-dependent synthetic lethality in BRCA1/2 -mutant cancers
Article Snippet: ADAR1 (p110, p150) - WB - Cell Signaling Technologies #14175 1:1,000 ADAR1 (p110, p150) - IHC - Santa Cruz #sc-73408 1:700 ADAR1 (p150) - IHC - Bethyl Laboratories #A303-883A 1 :1,000 -Actin - WB - Sigma Aldrich #A1978 1:10,000 BRCA1 - WB - Cell Signaling Technologies #9010 1:1,000 BRCA2 - WB - Cell Signaling Technologies #10741 1:1,000 Casp3 - WB - Cell Signaling Technologies #9668 1:1,000 c-Casp3 (N175) - WB - Cell Signaling Technologies #9661 1:1,000 CCNA2 - IF - Abcam #ab181591 1:1,000 CGAS - WB - Cell Signaling Technologies #15102 1:1,000 CHK1 - WB - Cell Signaling Technologies #2360 1:1,000 p-CHK1 (S345) - WB - Cell Signaling Technologies #2341 1:1,000 eIF2 - WB - Cell Signaling Technologies #5324 1:1,000 p-eIF2 (S51) - WB - Cell Signaling Technologies #3398 1:1,000 Fibrillarin - IF - Abcam #ab166630 1:4,000 G3BP1 - WB, IF - Cell Signaling Technologies #61559 1:1,000 GAPDH - WB - Cell Signaling Technologies #2118 1:1,000 -H2AX (S139) - WB, IF - Merck #05-636 1:1,000 IRF3 - WB (Hs) - Abcam #ab76409 1:1,000 IRF3 - WB (Mm) - Cell Signaling Technologies #4302 1:1,000 p-IRF3 (S396) - WB (Hs) - Abcam #ab76493 1:1,000 p-IRF3 (S396) - WB (Mm) - Cell Signaling Technologies #29047 1:1,000 LGP2 - WB (Hs) - Cell Signaling Technologies #12869 1:1,000 LGP2 - WB (Mm) - Abcam #67270 1:1,000 MDA5 - WB - Cell Signaling Technologies #5321 1:1,000 c-PARP1 (N214) - WB - Cell Signaling Technologies #9141 1:1,000 PKR - WB - Cell Signaling Technologies #12297 1:1,000 PKR - IF - Santa Cruz #sc-6282 1:500 p-PKR (T446) - WB - Abcam #ab32036 1:1,000 RAD51 - IF - Abcam #ab133534 1:1,000 RIG1 - WB - Cell Signaling Technologies #3743 1:1,000 RNase H1 - WB - Santa Cruz #sc-376326 1:500 RNA:DNA hybrids - IF - Kerafast #ENH001 1:500 RPA - IF - Abcam #ab2175 1:1,000 STAT1 - WB (Hs) - Cell Signaling Technologies #9176 1:1,000 STAT1 - WB (Mm) - Cell Signaling Technologies #14994 1:1,000 p-STAT1 (T701) - WB - Cell Signaling Technologies #9167 1:1,000 Unless indicated otherwise, all antibodies used in this study were validated externally by the suppliers for each corresponding application.
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